Our new Very clear+ tissue clearing procedure is the only real method that delipidates samples with no modify in morphology and with nominal influence on structural integrity.
Megatome is a vibrating microtome made to segment a broad variety of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With significant blade vibrating frequency and minimized blade deflection, Megatome permits high-throughput tissue sectioning with uniform surface profile, and also small tissue destruction and information reduction.
Antibodies may possibly acquire months to diffuse by only some millimeters of tissue, that has a steep labeling gradient from area to Main.
Megatome is created for accuracy: the blade vibrates at a higher frequency and larger amplitude assortment than other microtomes, and includes a special deflection Handle mechanism.
SE makes use of a rotational electrical area to disperse extremely electromobile molecules (like antibodies or surfactant micelles) all over a porous sample without having damaging electrically charged buildings in the tissue. This permits 2-4 working day clearing of intact organs,
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Megatome is really a novel microtome which allows for prime-precision sectioning of a wide array of tissue samples – from organoids, to arrays of animal organs, to intact human Mind hemispheres – with negligible tissue hurt and information decline.
eFLASH is actually a rapid tissue labeling approach that allows for uniform total-organ staining in 20 rounds of labeling.
Entirely delipidate whole mouse brains or comparably sized samples in only one working day with SmartBatch+, or in one 7 days with our passive clearing kit.
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SHIELD avoids the variability of hydrogel embedding and the knowledge decline from PFA preservation, protecting specimens for several rounds of processing.
Leverage the Crystal clear+ tissue clearing method, in addition to eFLASH and patented stochastic electrotransport systems, to promptly apparent and label whole organs. Crucial highlights and characteristics involve:
Our novel SHIELD tissue preservation method kinds intramolecular bonds employing polyfunctional, versatile epoxides to stabilize tissue architecture and safeguard the sample’s megatomi.com endogenous fluorescence, protein antigenicity and nucleic acids.